鱼菜共生养殖模式对水体微生物群落结构和红罗非鱼氨基酸组成的影响

打开文本图片集

Comparison of systematic microbial community structure and amino acid composition of red tilapia between aquaponics culture and traditional pond culture mode

LI Fangxi1 ， GAO Peng1，WANG Fengyu² ，DONG Xiaojing1

（1. College of Animal Science and Technology， Yangzhou University， Yangzhou 225O09，China;2. Lookess & Ecofarm，Lim Chu Kang ，Singapore）

ABSTRACT： In order to studythediference of microbialcommunity structure and aminoacid compositionofback muscle of red tilapia（Oreochromis spp.）between aquaponics culture（YC）and traditional pond culture（WT），we employed the sequencing platform to carry out high-throughput sequencing on the 16S rRNA gene of microorganisms present in water bodiesand utilizedahigh-performance liquidchromatograph toanalyze and determinethe aminoacid composition within the muscletisues of tilapia under two distinct aquaculture models. Compared to the WT group，the YC group displayed asignificantly higher microbialabundance and diversityin the waterbody.The dominant bacterial phyla included Actinobacteriota，Proteobacteria，Fusobacteriota，Bacteroidota，and Bdelovibrionota.The main dominant genera include Ceto bacterium，Flauobacterium，Aurantimicrobium，Rhodoluna，andCandidatus.Amongthecommondominantgenera，therelative abundance of Cetobacterium in the YC group was significantlyhigher thanthatin the WT group，while therelative abundance of Aurantimicrobium was significantly lower than that in the WT group. Being compared to the WT group，the YC group showed a marked increase in the ratios of total essential amino acids（£EAA）to total amino acids（£AA） and ∑EAA to total non-essential amino acids （£NEAA），while theratioof total umamiamino acids（∑DAA）to∑AA exhibitedasignificant decrease.Theaquaponicsculturehasa more diverse microbial community thanthe traditional pond culture and can improve red oreochromis's dorsal muscle qualityand increase its amino acid nutritional value to some extent.

KEY WORDS： aquaponics； microbial; bacterial diversity； red tilapia; amino acid composition

红罗非鱼（Oreochromis spp.）是尼罗罗非鱼和莫桑比克罗非鱼杂交的优良品系，自 20 世纪70 年代引进以来，因其肉质鲜嫩、产量高兼具观赏性，已成为热门养殖产品，价格也居高不下[1]。（剩余18034字）