神经介素B受体和促激素蛋白在猪垂体中的分布

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中图分类号：S828 文献标志码：A 文章编号：1671-4652（2025）06-0041-08

DOI：10.16872/j.cnki.1671-4652.2025.06.005

The distribution of neuromedin B receptor and tropic hormone protein in porcine pituitary

LI Wenqian1 ，MA Zhuol²，LI Hongxia¹ ， CHEN Guangzhen1 ，WANG Dandan1，LI Jia1，WANG Yiwen1，MA Zhiyu

（1. Jiangsu Co-Inouation Center for Prevention and Controlof Important Animal Infectious Diseases and Zoonoses/ College of Veterinary Medicine， Yangzhou University， Yangzhou 225Oo9， China; 2. Ningxia Vocational and Technical College for Minorities ， Wuzhong 751loo， China）

ABSTRACT： In order to detect the distribution and localization of NMBR and TSHβ ，ACTH， FSHβ ！ LHβ proteins in porcine pituitary，parafin sections of porcine pituitary tissue were prepared. The distribution of NMBR， TSHβ ，ACTH， FSHβ and LHβ proteins in porcine pituitary was detected by immunohistochemistry. The co-localization of NMBR and （204号 TSHβ ，ACTH， FSHβ ， LHβ proteins in porcine pituitary tropic hormone cells was detected by immunofluorescence. The results showed that in porcine pituitary tissue，NMBR-ir was widely distributed inthe adenohypophysis，and there were strong positive cells in the middle， nodular and distal marginal areas of the adenohypophysis. TSHβ -ir was mainly distributedin the pituitary nodulesanddistal （dorsal and ventral）.ACTH-irwas distributed intheadenohypophysis，butthe strong positivecels were mainlylocatedinthe ventraland lateralsidesof thedistalpartof theadenohypophysis.The distribution patterns of FSHβ -ir and LHβ -ir were similar，and the strong positive cells were mainly distributed in the adenohypophyseal nodules and the distal dorsal side. In addition，NMBR-ir and TSHβ ir，ACTH-ir， FSHβ -ir， LHβ irwere colocalizedin theadenohypophysis.Insummary，this studyclarified thedistributionof NMBRand four tropic hormone proteins in porcine pituitary from the morphological aspect，and found that NMBR existed in tropic hormone cels，which laid a foundation for the follow-up study of the role of NMB/NMBR system in porcine pituitary tropic hormone production.

KEY WORDS： porcine; neuromedin B receptor; tropic hormone; pituitary; distribution

垂体（pituitary）是动物最重要的内分泌腺之一，包括神经垂体（neurohypophysis）和腺垂体（adenohypophysis）2部分，主要通过激素来调节机体的新陈代谢和各项生命活动[1-3]。（剩余15589字）