基于circCOLIA1过表达和敲减的长江三角洲白山羊毛囊干细胞circRNAs测序及分析

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中图分类号：S858.26 文献标志码：A

Sequencing and analysis of circRNAs in hair follicle stem cells of Yangtze River Delta White Goat based on circCOLlA1 overexpression and knockdown

XUE Gang1，²，YU Xiaorong，SHI Jianfei1，JIANG Feng1

WANG Jian² ， WU ，GU Jie²，SHI Bo2 ， LI Yongjun²

（1.Instituteof Yangtze River Delta White Goat Breeding，Haimen District，Nantong City，Nantong 2261oo，China; 2. Jiangsu Key Laboratory of Animal Genetics ， Breeding and Molecular Design/ College of Animal Science and Technology ，Yangzhou University Yangzhou 225oo9， China）

ABSTRACT：The Yangtze River Delta White Goat，also known as the Haimen Goat，is a famous goat bred in China and the onlyone that can produce high-quality writing brush hair（type Ilhair）．circCOLlA1has been confirmed to play an importantrole in the formationof high-quality writing brushhair.This study further focusedoncircCOL1Al，performed cirular RNAs （circRNAs）sequencingonhair follcle stemcelswith cirCOLAl overexpressionand knockdown，andanalyzed thefunctionsofdiferentialcirRNAsandtheirsourcegenes.Functionalenrichmentandinteractionnetworkanalysesshowedthat diferentiallexpresedcirRNAssuchascircACTN1，circTGA6andcirCOBLLimportantregulatoryroles inhairfollilede velopment（includingthegrowthanddevelopmentofhairfoliclestemcels）andtheformationofhigh-qualitywritingbrushhair， exerting their important regulatory efects through circITGA6/miR-18a-5p-FGF1/MAP3K1 axis such as the circACTN1-miR671-5p-MAPK3/COL13A1/KRTAP3 axis and the circITGA6-miR-18a-5p-FGF1/MAP3K1 axis.

IEY WORDS： goat； superior-quality brush hair; circCOL1Al gene;hair follicle stem cells；regulatory mechanism长江三角洲白山羊又称海门山羊或笔料毛山羊，是我国唯一一个能生产优质笔料毛的著名山羊品种[1]。（剩余16606字）