丁香酚对南方根结线虫的熏杀作用机制

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中图分类号：S432.45 文献标志码：A

1.海南大学热带农林学院/热带农林生物灾害绿色防控教育部重点实验室，海南海口570228；2.儋州入侵生物海南省野外科学观测研究站，海南儋州571737；3．海南南繁种业集团有限公司，海南三亚572025

Fumigation Mechanism of Eugenol on Meloidogyne incognita

LIAO Yuqi1， CHEN Zongxiong13， HUI Renjie1， YU Zilin1， HUANG Yekai1， DING Xiaofan1，2"

1.SchoolofTropical AgricultureandForestry，HainanUniversity/KeyLaboratoryofGeenPreventionandControlofTropical Plant Diseasesadests，iistryofEducationHaikou，Hainan57o8，China;2.anzouIvasiveSpeciesObservatiodRe searchStationofHainanProvce，Danzou，Hainan57737，China;3.HainanNanfanSedIdustryGroup，anya，Haian57025， China

Abstract： Meloidogyne incognita isoneof the most destructive plant-parasitic nematodes （PPNs）in the world，which poses a serious threat to many important economic crops and causes huge agricultural economic losss every year. In this study，the plant-derived compound eugenol was used as the research object to exploreits mechanism offumigation against M incognita.The results of in vitro fumigation bioassay showed that eugenol had significant inhibitory activity against the second instar larvae （J2s） of M☉ incognita，and its LC50 value was 123.453μg/mL . Structural observation showed that after treatment with 800μg/mL eugenol for 24h ，thestructural integrity of J2s body wall was destroyed， which was manifested as body surface relaxation，bodyring enlargement， inclusion leakage and significant vacuolization，and abnormal expansion of esophageal gland.The physiological and biochemical analysis further revealed that eugenolcould cause body fluid extravasation and electrical conductivity increase of J2s and interfere with its osmotic regulation and respiratory metabolism proces.In addition，it also significantly afected the activityof many key enzymes in M. incognita J2s， including inhibiting detoxification function， disrupting antioxidant defense system and interfering with nerve signal transduction and causing nutrient metabolism disorders.The results showed that eugenol ultimately led to thedeath of nematodes bydestroying structural integrity，interfering with physiological metabolism and energy supply.The findings would provide atheoretical basis for the development of eugenol as an environmentally friendly fumigant.

Keywords： Meloidogyne incognita; eugenol; mechanism; physiological and biochemical DOI：10.3969/j.issn.1000-2561.2026.04.019

植物寄生线虫（plant-parasiticnematodes，PPNs）是具破坏性的农作物病原，在全球范围内，其每年造成的经济损失高达1570 亿美元[1-2]。（剩余18138字）