OsASR5调控水稻苗期耐盐性的功能分析

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中图分类号：S511 文献标志码：A

Functional Analysis of OsASR5 in Regulating Salt Tolerance at the Seedling Stage in Rice

HE Yuxin1，23，BAI Liangli4， HU Xianlan4， WANG Jiao4， LI Yaokui²， TANG Li124*

1.SchoolofopicalAgricultureandForestryHaianUniversityDnzou，Hainan57China;2.tateKeyLaboatoyf HybridRice，HunanHybridRiceResearchCenter，Changsha，Hunan40l5，China;3.Natioal InnovationCenterforSalttolerance Rice Technology，Sanya，Hainan57o19，China;4.LongpingAgricultureCollge，HunanUniversity，Changsha，Hunan41015， China

Abstract： Abscisic acid-，stres-，ripening-induced protein （ASR）playsanimportantrole inplantresponse toboth biotic and abiotic streses.Inthisstudyweconductedbioinformaticaalysisonthe membersofthericeASRgenefamilyclonedthe OsASR5 gene and analyzed its expression patterns in diferent tissues and under salt stress，and generated osasr5 knockout lines to investigate the function ofOsASR5 in regulating salt tolerance during rice seedling stage. Bioinformatics analysis showed that rice had six ASR members，locatedonchromosomes1，2，4and11.Except for OsASR2，the promoterregions of other familymembers allcontained ABAresponse elementsand encoded hydrophilic proteins，Expresionanalysis indicated that OsASR5was highly expressed inleaves and was rapidly induced bysalt stressPhenotypic analysis indicated that compared with the wild type，osasr5 knockout lines exhibited reduced sensitivityto exogenous ABAbut increased sensitivityto salt stress.Under seedling-stage stress， the shoot Na+ content and Na+/K+ ratio of knockout lines increased significantly， while ABA and proline contents decreased markedly， and hydrogen peroxide （H2O2） and malondialdehyde （MDA） contents elevated. In summary，this studyreveals the basiccharacteristicsof the ASR gene family inriceand demonstrates that OsASR5 positively regulates saltleranceat the seedling stage by promoting ABAand prolineaccumulation in shoots，scavengingreactive oxygen species， and reducing Na+ content in shoots. The findings would lay a foundation for further elucidating the moleculal mechanisms of OsASR5 in response to salt stress.

Keywords： salt stress; Oryza sativa L.; OsASR5; physiological indicators DOI：10.3969/j.issn.1000-2561.2026.04.004

盐胁迫是制约全球农作物产量的关键非生物胁迫因子之一。（剩余19409字）