橡胶树HbERF109互作蛋白筛选

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中图分类号：S794.1 文献标志码：A

Screening of Interacting Proteins for HbERF1o9 in Rubber Tree （Hevea brasiliensis）

WEN Wei1.2， JIANG Weizeng1，2， YUAN Hongmel1，2*

1.SchoolofTropical AgricultureandForestry（AgricultureandRuralColege，RuralRevitalizationCollge），HainanUnivesity/ NaturalRubberProvincialandMinisterialColaborativeIovationCenter，anya，Hainan572O5，Chia;2.SanyaIstuteof BreedingandMultiplication，HainanUniversity，Sanya，Hainan572o25，China

Abstract： The transcription factor ERF109，a member of the AP2/ERF subfamily，plays a pivotal role in plant stress responses.To further elucidate the molecular mechanism of HbERF109 in Hevea brasiliensis under low-temperature stress and exploreitspotential regulatorsin stress resistance，the structureof HbERF109 wascharacterized，its auto-activation activityeliminated through protein truncation，and interacting proteins were screened usinga yeast two-hybrid （Y2H） library. Candidate interactors were further validated by bimolecular fluorescence complementation （BiFC）assays.The ful-length HbERF109 exhibited strong auto-activation activity，which was abolishedafter truncation of the first 83 amino acids at the N-terminus （△N）. Using the constructed bait vector pGBKT7-HbERF109-△N for library screning，a totalof30 potential interacting proteins，including HbSODand HbAPX， were identified.The proteins are functionallyimplicated inphotosynthesis，material transport，geneexpressonregulation，stressresponse，and disease defense.Yeast two-hybrid and BiFC assays further confirmed the interactions between HbERF109 and HbSOD as wellas HbAPX.Colectively，the findings indicate that HbERF109 mayfunction as an integrative regulatory component in the low-temperature stress response of rubber tree by coordinating a multi-layered protein interaction network associated with photosyntheticperformance，antioxidant capacity，and watertransport.This study would provide new insights into the molecular mechanism through which HbERF109 contributes to the stress resistance of Hevea brasiliensis. Keywords： Hevea brasiliensis; yeast two-hybrid; superoxide dismutase; ascorbate peroxidase; interacting protein DOI： 10.3969/j.issn.1000-2561.2026.04.001

橡胶树（Heveabrasiliensis）又名巴西橡胶树，属大戟科橡胶树属，是典型的热带植物，原产于南美洲亚马逊河流域。（剩余18579字）