香芹酚通过磷脂酰肌醇3-激酶途径诱导牙周炎牙槽骨修复的机制研究

打开文本图片集

[中图分类号] R781.4[文献标志码]A [doi]10.7518/hxkq.2025.2025050

[Abstract]ObjectiveThis study aims to explore the mechanism of carvacrol （CV） in regulating alveolar bone repair in periodontal disease.MethodsArat modelof periodontal disease （Pgroup）was created byligatingthe first molar and injecting lipopolysaccaride（LPS）.Acontrol model（C group）was also created.The treatment models received low （L group）， medium （M group），and high （H group） doses of CV hydrogel. Hematoxylin-eosin （HE） staining was used to observe the pathological changes in periodontal tsues.Immunohistochemical staining was employed to analyze the expressionof collagen type I（COL1）andRunt-related transcription factor2（Runx2）.Invitro，therat osteoblast cells were divided into C，P，L，M，H，CVand CV+LY294002（CV+LY） groups. Western blot analysis detected the phosphatidylinositol 3-kinase （PI3K）/protein kinase B （AKT）/glycogen synthasekinase3 beta（GSK-3β）pathway-related and osteoblastic proteins.Quantitative reverse-transcription polymerasechain reactionwasused tomeasure the expressionof inflammatory factors and osteoblastic proteins.The alkaline phosphatase （ALP）colorimetric kit andalizarin redS staining kit were utilized to assess osteogenic ability. Immunofluorescence （IF） was used to detect COL1 expression in osteoblasts. Transmision electron microscopy was applied to detect cellapoptosis.ResultsCVhydrogel aleviated periodontal symptoms，upregulated PI3K/AKT/GSK- ⋅3β （2 pathway-related and osteoblastic proteins，and increased theexpresionofALPand the number ofcalcified nodules. However， it decreased cellapoptosisand inflammatory factors.LY294002 inhibited the PI3K/AKT pathwayand decreased osteoblastic proteinexpresion，ALPcoloration，andcalcified nodule quantity.ConclusionCVhydrogel pro motes theproliferationanddiferentiation of alveolar bone osteoblasts byactivating the PI3K pathwayand inhibiting inflammation-inducedboneresorption.ThisstudyemphasizesthepotentialofCVforthetreatmentofperiodontaldiseases.

[Key words] carvacrol;periodontal disease；osteoblasts；phosphatidylinositol 3-kinase；lipopolysaccharide

牙周炎是一种常见的慢性炎症性疾病，对人类的健康和生活质量造成显著影响[。（剩余17717字）