丹参种根和愈伤组织保存方式研究

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中图法分类号：S567.23 文献标识码：A 文章编号：1000-2324（2026）01-0149-08

Abstract：This study investigates the effects of low-temperature preservation at 4∘C ontheviabilityofSalviamiltiorrhiza seed roots andcalls，andanalyzes therelationshipbetweenpreservation medium，rootsegment size，preservationduration and root germination rate，aiming to provide technical basis for the medium-and long-term preservation of S. miltiorrhiza germplasm resources. Using fresh root segments of different sizes and primary calli from one-year-old S. miltiorrhiza as materials，thisexperiment divides therootsegments into four groups basedonlengthanddiameter，determiningtheseedroot germinationrate bythesand culture method，and evaluating calus viabilitythrough survivalrateofcali during subculture. The results show that wet sand preservation at 4°C effectivelymaintains the seed root quality，significantly inhibits premature germination，preserves watercontent and viabilityand yields ahigh germinationrate.Root segment lengthanddiameter exertasignificant effecton sedling establishmentrate after low-temperature preservation.Most seed roots maintain a seedling establishment rate above 85% within one year of low-temperature preservation，and with increases in the length and diameter of the S.miltiorrhizaroot segments，both the sedling emergencerate and seedling height correspondinglyrise. Calli retain seedling formation capacity after 9～12 months of preservation at 4∘C ，although variations in preservation duration tolerance and viability exist among diferent varieties.Insummary，low-temperature wet sand preservationat 4∘C （204号 allows large-dimensionS.miltiorrhizaseedroots tobestoredforuptooneyear，providingafeasibleapproachtoreduce the costof biennialplanting.Additionally，Cali maintainstrong seedling formationcapacitywithin9monthsoflow-temperature preservation，offering a basis for reducing subculture frequency and simplifying the preservation protocols.

Keywords： Salvia miltiorrhiza; low-temperature preservation; root segment; callus

药用植物种质资源是持续培育优良新品种、不断提高中药材质量的重要物质基础。（剩余9881字）