山东省小麦茎基腐病菌毒素化学型鉴定及其致病力相关性分析

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Identification of Toxin Chemotypes of Fusarium pseudograminearum in Shandong Province and Analysis of Their Correlation with Pathogenicity

BI Zhuo-yu， ZHANG Ren-jie*， ZHAO Chen， WU Zhen， SONG Jia-le， WU Yue-ming， YU Jin-feng， ZHANG Li**， CUI Hai-tao*

CollegeofPlantProtection/ShandongAgricultural University，Tai'an271o18，China

Abstract：Theoccurrenceofwheat crownrothas become increasinglysevere in China's major wheat-producing regionsand hasemergedasamajor disease threatening food security.Fusariumpseudograminearum，oneoftheprimary pathogens responsible forthis disease，exhibits a pathogenic mechanism closely associated with the production of mycotoxins，among which deoxynivalenol （DON） and nivalenol （NIV） serve as key pathogenic factors. Using 125 strains of F. pseudograminearum strains from8regionsof ShandongProvinceas materials，this studyconducts PCRwith specific primers tosystematically analyzethe toxinchemotypes of the pathogen.Results show that15-AcDON-producing strainsaccounts forthe highest proportion at 56% （70/125）， followed by 3-AcDON-producing strains at 38.4% （48/125），while NIV-producing strains account for the lowest proportion at 5.6% （7/125）.Pathogenicity assessments show differential virulence among toxin chemotypes，with NIV-producing strains exhibiting thestrongest pathogenicity.Furthermore，DON productionanalysis indicates that 3-AcDON-producing strains exhibitsignificantlyhigher toxin synthesis capacity than15-AcDON-producing strains，andtoxinlevels haveapositivecorrelationwith pathogenicityonwheatcoleoptiles.Thisstudyelucidatesthetoxin chemotype composition of F. pseudograminearum in Shandong Province and their relationship with pathogenicity， providing critical data support and a theoretical basis for integrated management of wheat crown rot.

words：Fusarium pseudograminearum; toxin chemotype; NIV; 3-AcDON;15-AcDON; pathogenicity

小麦茎基腐病是目前小麦生产上最具毁灭性的土传病害之一，严重威胁我国小麦品质和粮食安全。（剩余11152字）