97份特色小麦种质资源萌发期耐盐性筛选与鉴定

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Abstract：Toevaluatethesalttoleranceof97characteristicwheatgermplasmresourcesat thegerminationstage，thisstudy sets up a150 mmol L-1ΔNaCl treatmentent and a control （distilled water），and measures the germination rate，bud length，root length， fresh weight，and dry weight on the 7th day using the Petri dish culture method.It calculates the comprehensive salt tolerance index （D-value）bycombiningthemembership functionmethodandweighted principal component analysis PCA） method.Then this studyperforms cluster analysis basedontheD-values toevaluate theefectofsalt solutionon the germination of characteristic wheat and to scren for salt-tolerant germplasmresources.Theresults show that salt stress leadstoreductionsinthegerminationrate，budlength，rootlength，freshweight，anddryweightofcharacteristicwheat varieties. The cumulative contribution rate of the three principal components is 92.55% .The five indicators correlate closely withthesalttoleranceofcharacteristicwheatatthegerminatiostage，andcanserveasevaluationcriteriaforassessngsalt tolerancein characteristic wheat atthe germination stage.Thesalt tolerancecapacityvaries significantly among different germplasm resources，with D-values ranging from -2.26 to 4.54.Cluster analysis classifies the 97 characteristic wheat germplasmresources into five grades：one high salt-tolerant germplasm，twosalt-tolerant germplasms，ten moderatelysalttolerant germplasms，82salt-sensitive germplasms，andtwoextremelysalt-sensitivegermplasms.This studyprovidesa technicalframeworkandfoundational materialsforlarge-scalesalt-toleranceidentificationofcharacteristicwheat germplasm resources at the germination stage and for the cultivation of new salt-tolerant varieties.

eywords： wheat; germplasmresources; germination stage; salt tolerance; principal component analysis; cluster analy

我国是世界上盐碱地分布最广的国家之一，盐碱地总面积约为9913万 hm2 ，主要集中分布在东北、西北、华北及滨海地区[1。（剩余10923字）