4种食品基质中双丙酚丁、双辛酚丁和双醋酚丁的高效液相色谱-串联质谱法测定
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中图分类号：TS255；O657.63 文献标志码：A 文章编号：1671-4652（2026）01-0128-1（
Determination of dipropylphenyl butyrate， dioctylphenyl butyrate and diacetylphenyl butyrate in four food matrices by high-performance liquid chromatography-tandem mass spectrometry
CHEN Liangqian 1，2 ，WU Yuehaol ，ZHANG Lu²，GE Qingfengl （1. College of Food Science and Engineering，Yangzhou Uniuersity， Yangzhou 225127，China; 2. Nanjing Product Quality Supervision and Inspection Institute （Nanjing Institute of Quality Development and Aduanced Technology Application），Nanjing 21oo28， China）
ABSTRACT：Todevelopasensitive and matrix-compatible high-performance liquid chromatography-tandem mass spectrometry （HPLC-MS/MS）methodforqualitativeandquantitativedeterminationof bispyrbenzylanalogs-dipropylphenolphthalein（DPB）， dioctylphenolphthalein（DOB）anddiacetylphenolphthalein（DAB）indiversefood matrices.Samples wereultrasonicallextracted with methanol and separated on an Agilent SB-C18 column （100mm×2.1mm×3.5μm） using a 0.1% formic acid water-0. 1% formic acid acetonitrile gradient at 0.4mL⋅min-1 （ 5μL injection）. Detection was performed by ESI in MRM mode with external calibration.Method validation covered linearity， limits of detection （LOD）/limits of quantification （ LOQ） ，recovery/precision and matrix effect （ME）. The results showed that excellent linearity was obtained over 1-50ng∙mL-1 （ R2>0.999 ）.Under 1g sample， 25mL dilution and 5μL injection，LOD and LOQ were 25 and 75μg⋅kg-1 ，respectively. Spike recoveries across jelly，candied fruit， solid beverage and gummy matrices ranged from 86.2% to 104.6% with relative standard deviations of 1.02%-6.32% . ME values were within 93.5%-98.6% ， indicating negligible suppression/enhancement. In theconclusions，the methodofers high sensitivity，robust anti-matrix performance，operational simplicityandreliabl quantification，enabling trace-level determination of DPB，DOB and DAB in multiple food matrices.
KEY WORDs： food matrices；buphenine；dioctylphenol butane；diacetophenol butane；high-performance liquid chroma tography-tandem mass spectrometry （HPLC-MS/MS）;determine
近年来，肥胖被世界卫生组织（WHO）列为全球性流行病，其防控已成为公共卫生领域的重大挑战。（剩余15628字）
