鸡RACKI基因克隆与生物信息学分析及其在鸡不同组织中的表达分析
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中图分类号：S831.2 文献标志码：A 文章编号：1671-4652（2026）01-0065-09
DUI： 10.1687Z/j.cnk1.1671- 465Z.Z0Z6.01.008
Cloning，bioinformatic analysis of chicken RACKl gene and tissue expression analysis in chickens
CAO Shukai1，XIA Lei²，PING Yuyu²，WANG Qiangzhou²，YIN Na2 ，BAI Hao²，CHEN Shihao （1. College of Animal Science and Technology， China Agricultural University， Beijing 10o193， China; 2. Institute of Epigenetics and Epigenomics， Yangzhou University， Yangzhou ， China）
ABSTRACT：This studyaims toinvestigate the biological characteristicsof thechicken receptor foractivated Ckinase1 （RACK1） protein，assess its role in infectious bursal disease virus （IBDV）replication，and examine its expression patterns acrossvarious chicken tisues.TheRACKl gene from chickens was cloned，and structural and functional predictions of the encoded protein were performed using various bioinformatics tools.A eukaryotic expression vector carrying chicken RACK1 gene wasconstructed，and itsexpression was verified byWestern blot.TheefectofRACK1overexpression on IBDVreplication was also evaluated.Furthermore，the relative expression levels of RACK1 in different chicken tissues were determined byqRT-PCR.The study revealed that chicken RACK1 consistedof317aminoacid residues，with a molecular mass of approximately 34.87ku . The protein lacks transmembrane domains and signal peptide but contained seven typical WD4O domains. Its secondary structure was primarily composed of random coils （36.59% ）and extended strands （45.11% ）.Protein-protein interaction predictions suggested that RACK1 may interact with ribosomal proteins such as RPL38，RPL27A，RPL23，RPLPO，and RPS2.Phylogenetic analysis demonstratedthatRACK1 was highlyconserved across several species. The pCAGGS-HA-chRACK1 eukaryotic expression vector was successfully constructed and confirmed tobeexpressed incells.Inan IBDV infection model，RACK1overexpression significantly enhanced viralreplication.qRT-PCR analysisof diferent chicken tisses revealed thatRACK1expresson was highest inthe heartand lowest in the spleen.In conclusion，this study characterizes the molecular structure and tissue distribution of chicken RACK1 anddemonstrates its positive regulatoryrolein IBDV replication，providing a theoretical basis for further research into RACK1 function in avian viral infections and immune responses.
KEY WORDS： chicken; RACK1 gene; eukaryotic expression vector; bioinformatics analysis; tissue expression
活化蛋白激酶C1受体（receptor of activated protein kinase C1，RACK1）属于含有色氨酸-天冬氨酸（WD）重复结构的蛋白质家族。（剩余14520字）
