蒺藜首蓿NAC转录因子基因MtNAC47克隆及表达分析
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中图分类号：S326 文献标识码：A 文章编号：1673-9868（2026）02-0098-13
Cloning and Expression Analysis of the NAC Transcription Factor Gene MtNAC47 in Medicago truncatula
YANG Chun1， SHEN Liangying1， LI Shuwen²， JIA Wei1， CHAO Yuehui1 1.School of Grassland Science，Beijing Forestry University，Beijing100083； 2.Beijing Middle School，Beijing 100018
Abstract： Plant NAC proteins constitute a crucial family of transcription factors involved in plant growth， development，and stress responses. This study cloned the MtNAC47 gene from Medicago truncatula 4 R108， and preliminarily investigated its function through bioinformatics analysis，subcellular localization， transcriptional self-activation assays，and expression pattern analysis. The results showed that the fulllength of MtNAC47 gene was 1 023bp ，encoding 34O amino acids，and it exhibited the closest phylogenetic relationship with TpNAC47 from Trifolium pratense. Subcellular localization revealed that MtNAC47 was localized in both the nucleus and cytoplasm. Yeast self-activation assays demonstrated that MtNAC47 possessed self-activation activity. Expression pattern analysis indicated that the expression levels of MtNAC47 in diffrent tissues of Medicago truncatula were sequentially as follows： flowers，roots， stems，leaves，and pods. Abscisic acid（ABA），methyl jasmonate（MeJA），salicylic acid （SA），gibberellic acid（GA），and low-temperature stress all regulated the expression of MtNAC47. Under ABA，SA， and GA treatments，expression levels initially increased and then decreased. MeJA treatment inhibited expression，while low-temperature stress caused an initial decrease followed by an increase. These findings suggest that the MtNAC47 gene may play a significant role in plant growth and development，as wellas in environmental stress responses.
Key words： Medicago truncatula ； MtNAC47 ； self-activation detection； subcellular localization; expres-sion pattern analysis
转录因子（TranscriptionFactor，TF）是一类通过与特定顺式作用元件结合来调控基因表达的蛋白质，在植物应对非生物胁迫、激素信号转导以及生长发育过程中发挥着重要作用[-2]。（剩余16038字）
