中华按蚊拟除虫菊酯抗性相关P450基因的变异和进化研究

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中图分类号： log69.44+2.2 文献标识码：A

mission in China，and its resistance to pyrethroid insecticides has become a major challnge for malaria control efforts. Based on the resequencing data from 36An . sinensis samples and reference genome data， variant detection was performed using BWA and GATK software. Single nucleotide polymorphism （SNPs） data of P450 genes were extracted， folowed by phylogenetic tree analysis， principal component analysis （PCA），population structure analysis，and genetic variation analysis. This study explored the single nucleotide polymorphisms and evolution of P450 genes associated with pyrethroid resistance in An . sinensis at the genomic level. The results showed that SNPs and InDels in the P450 genes of An . sinensis were mainly distributed in intergenic regions. Population structure analysis revealed that the 36 samples could be divided into three genetic groups，with potential geographical isolation among the populations. The Chongqing population exhibited a scattered clustering patern. Genetic variations were identified in the （204号 P450 genes CYP6P3γl，CYP9J10 ，and CYP9K1，primarily concentrated in the CYP6 and CYP9 families，as corroborated by previous expression profiling and RNA interference experiments. Specifically， CYP6P3v1 contained a synonymous SNP（Ll18L） in the coding region and intronic SNPs，CYP9J10 exhibited a nonsynonymous SNP（S162N） in the coding region，and CYP9K1 mainly had SNPs in intergenic regions. The clustering of the 36An . sinensis samples in this study aligned with their geographic distribution， suggesting potential geographic isolation among different populations. Furthermore，the genetic variations in CYP6P3vI ，CYP9J10，and CYP9K1 identified in this study may be associated with pyrethroid resistance.

Key words： Anopheles sinensis ;P45O gene; SNPs; InDels

中华按蚊 Anopheles sinensis 隶属节肢动物门（Arthropod）、昆虫纲（Insecta）、双翅目（diptera）、蚊科（Culicidae）、按蚊属Anopheles，是疟疾、马来丝虫病、乙型脑炎等疾病的重要媒介蚊虫，在我国和东南亚国家广泛分布[1-3」。（剩余16898字）