O型口蹄疫病毒VP1结构蛋白的原核表达与免疫原性

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中图分类号：S855.3 文献标志码：A 文章编号：1004-390X（2025）06-0060-08

Abstract： [Purpose] To construct the recombinant plasmid pET-28a-His-VPl for expressing the VP1 protein of type O foot-and-mouth disease virus （FMDV）. The expressed VP1 protein was purified and used to immunize mice to analyze its immunogenicity， aiming to provide theoretical and experimental insights for the development of FMDV subunit vaccine. [Methods] Expression conditions for VPl protein were optimized by isopropyl- ⋅β -D-thiogalactopyranoside （IPTG） concentration， induction temperature， and induction time. Serum antibody titers and subtypes were determined by indirect ELISA. A serum neutralization test was performed using BHK-21 cels to assess protective efficacy， and mRNA expression levels of cytokines were quantified by qPCR. [Results] VP1 protein expression was achieved by induction with 0.80mmol/L IPTG at 37∘C for 10h . Immunization with the VP1 protein triggered a strong humoral immune response， with an antibody titer reaching 1：409600 ， and induced a mixed Th1/Th2 immune response， as indicated by the presence of IgG2a， IgG2b，and IgG1 subtypes. VP1 immunoserum exhibited good neutralizing activity， which could effectively inhibit viral adsorption. Additionally， VP1 immunization significantly upregulated the mRNA expression of IL-6 and TNF- a [Conclusion] The recombinant VP1 fusion protein is successfully expressed in Es cherichia coli with high immunogenicity， ofering a promising candidate antigen for FMDV subunit vaccine design.

Keywords： type O foot-and-mouth disease virus; VPl structural protein; immunogenicity; subunit vaccine

口蹄疫（foot-and-mouth disease，FMD）是由□蹄疫病毒（foot-and-mouthdiseasevirus，FMDV）引起的一种感染牛、羊、猪等偶蹄动物的烈性接触性传染病[1]。（剩余13729字）