1株杉木赤枯病菌拮抗细菌的筛选与鉴定

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中图分类号：S436.421.13 文献标志码：A 文章编号：1004-390X（2025）06-0033-10

Abstract： [Purpose] To provide strain resources and theoretical basis for the biocontrol of copper blight of Cunninghamia lanceolata caused by Pestalotiopsis microspora. [Methods] Strain SMTR-1， screened from the rhizosphere soil of healthy C lanceolata and showed high antagonistic activity against copper blight， was identified by morphological observation， physiological and biochemical determination and molecular biology methods; and its antibacterial effect was evaluated using the dual culture method （double dish method for volatile substances）， spore germination assay， and mycelial growth rate method. [Results] Strain SMTR-1 was identified as Pseudomonas aeruginosa. Its sterile filtrate，fermentation broth，and volatile substances all exhibited significant inhibitory effects on

P microspora.Among these， volatile substances showed the most significant inhibitory efect， with an inhibition rate of 79.86% . Microscopic observation revealed a teratogenic effect on the hyphae of P microspora，manifesting as distortion and deformation of pathogenic hyphae， sweling of some cells， varying thickness， and eventual rupture. [Conclusion] The results of this study provide microbial strain resources for the biological control of copper blight of C lanceolata and offer valuable reference for the research and development of related biocontrol agents and formulations.

Keywords： Pseudomonas aeruginosa; copper blight of Cunninghamia lanceolata; antagonistic spectrum; sterile filtrate; volatile substances; Pestalotiopsis microspora

杉木[Cunninghamia lanceolata （Lamb.） Hook.]属松柏目（Pinales）杉科（Taxodiaceae）杉木属（Cun-ninghamia）常绿针叶乔木，因其生长速度快、成活率高、材性优良、易于加工、具有药用价值等特点而广泛种植，是中国南方重要的速生用材树种和生态树种[1]。（剩余14031字）