黄瓜CsHLS2基因的克隆与表达分析

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中图分类号：S642.2：Q781 文献标识号：A 文章编号：1001-4942（2026）01-0017-10

AbstractHLS1 is a key gene regulating plant apical hook formation and is also involved in multiple plant developmental processes. CsHLS1 modulates plant architecture in cucumber by regulating the sizes of leaf and petiole angles.However，whether CsHLS1 has homologs in cucumber and their potential biological functions remained to be elucidated.In this study，a protein highly homologous to CsHLS1 was identified in cucumber by constructing a phylogenetic tree of CsHLS1，which was designated as CsHLS2. Furthermore， CsHLS2 from cucumber was cloned and its molecular characteristics and expression profiles were investigated.The results showed that the full-length sequence of CsHLS2 was 2 158bp ，encoding 278 amino acids. The CsHLS2 protein had the molecular weight of 32.25，the theoretical isoelectric point of9.O3，the instability index of 9.24，the aliphatic index of 84.17，the grand average of hydropathicity of -O.415，and had no signal peptide or transmembrane domain，indicating that it was a stable protein. The secondary structure of CsHLS2 was mainly composed of random coils （59.71%） ）and α -helices （ 26.26% ）. Phylogenetic analysis and conserved domain analysis revealed that CsHLS2 in cucumber had the closest genetic relationship with CmHLS2 in melon （Cucumis melo）.The promoter region of CsHLS2 was cloned and analyzed for its action elements，and the result demonstrated thatthis region contained multiple environmental and hormone-responsive elements.Quantitative real-time PCR（RT-qPCR）analysis indicated that CsHLS2 was highly expressed in cucumber ovaries and petiole bases.These findings could provide a foundation for further elucidating the functional rolesof CsHLS2 in cucumber growth and development.

KeywordsCucumber; CsHLS2; Gene cloning； Expresson pattern analysis

HOOKLESS（HLS1）为乙烯响应基因，编码一个N-乙酰基转移酶，其产物是顶端弯钩形成所必需的[1]，在植物生长发育的多个方面也起到重要作用，被认为是植物中的多功能分子。（剩余11781字）