甘蓝型油菜冷调节基因BnaCOR27的克隆及表达分析

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中图分类号：S634.3：Q781 文献标识号：A 文章编号：1001-4942（2026）01-0008-09

Cloning and Expression Analysis of BnaCOR27 Gene in Brassica napus

Peng Yangyu， Chen Yudie， Zhang Zerong，Hong Bo， Xing Man，Xiong Xinghua（College of Agriculture， Hunan Agricultural University， Changsha 41O128， China）

AbstractThe COR27 gene is a cold regulatory gene important in plant response to cold stress To explore the function of COR27 gene from Brassica napus，the variety of ZS11 was used and a COR27 full-length CDS sequence of 723 bp was cloned.， It encoded 240 amino acids and was named as BnaCOR27. Bioinformatics analysis showed that the BnaCOR27 protein was a hydrophilic protein with poor stability，had no transmembrane domain and signal peptide，and was a non-secreted protein. Multiple sequence alignment revealed low sequence identity between BnaCOR27 and COR27 proteins of other 17 species such as Brasica rapa，Hirschfeldia incana， and Raphanus sativus.The phylogenetic evolutionary tree showed that the COR27 proteins of Cruciferae were clustered together，and BnaCOR27 had the most close evolutionary relationship with COR27 of B.rapa.Promoter element analysis showed that the expresion of BnaCOR27 was not only induced by low temperature but also regulated by light signals，and it played an important role inresponse tocold stressaswellasother stresstresses. The results of real-time fluorescence quantitative PCR detection showed that BnaCOR27 had the expression pattrn of leaf > root > flower > stem > silique in B. napus ZS11. The overexpression vector pCAMBIA1301-BnaCOR27 was successfully constructed.These results could lay the foundation for in-depth exploration of the mechanism of BnaCOR27 gene in cold resistant process and provide a theoretical guidance for molecular breeding of B.napus.

KeywordsCOR27; Brassica napus L.; Cold stress; Bioinformatics analysis； Expression analysis;； Over-expression vector

甘蓝型油菜（BrassicanapusL.）是食用植物油的重要来源作物，种植面积及产量均位居世界油料作物前列，其营养价值高、分布广，是世界上种植最广泛的温带油料作物[1]。（剩余12228字）