丹参转录因子基因SmbHLH59的克隆与表达特征分析

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中图分类号：S567.53：Q781 文献标识号：A 文章编号：1001-4942（2026）01-0001-07

Cloning and Expression Features of Transcription Factor Gene SmbHLH59 from Salvia miltiorrhiza

Liu Cong'，Wang Chaoyang1，Liu Jiangbin1，Mei Xiangui 1 （20 Wang Chenchen²，Ren Zhenli²，Song Zhenqiao' （1. College of Agronomy， Shandong Agricultural University， Taian 271O18， China; 2. Shandong Danhong Pharmaceutical Co.， Ltd.，Heze 2740oo，China）

AbstractTanshinones are the primary bioactive compounds in Salvia miltiorrhiza，and their biosynthetic pathways are regulated by various factors.The basic helix-loop-helix （bHLH） transcription factor family plays crucial roles in plant growth metabolism，secondary metabolite synthesis，and stressresponses.To explore the role of bHLH in tanshinone biosynthesis，the full-length coding sequence （CDS）of SmbHLH59 was cloned fromS.miltiorrhiza，followed by comprehensive bioinformatics and expression profile analyses.The results showed that SmbHLH59 was conserved in structure，containing three exons and two introns.The SmbHLH59 protein was composed of 226 amino acids with weak acidity and highly hydrophobicity，and was a lipophilic protein.It was characterized by low stability，a high proportion of randomcoils，and high complexity.Subcellular localization predicted that SmbHLH59 was located in the nucleus.It had the closest relationship with bHLH126 from Citrus splendens.The promoter region of SmbHLH59 was enriched with cis-acting elements response to phytohormones.Expression analysis revealed that SmbHLH59 reached the highest expression level in phloem of the red rots and was significantly up-regulated when induced by methyl jasmonate（MeJA），which suggested that SmbHLH59 was involved in tanshinone biosynthesis in the red roots and was regulated by MeJA. This study could provide a vital foundation for further functional characterization of the SmbHLH59 gene.

KeyWordsSalvia miltiorrhiza； bHLH Transcription factor; Gene cloning;Bioinformatics analysis； Ex-pression analysis

丹参（SalviamiltiorrhizaBge.）是我国常见的大宗中药材，采收时间常于当年秋季或次年春季，净制干燥后切片入药，性微寒、味苦，归肝经和心经[1]，具有活血祛瘀止痛、凉血消痈等功效，在历代中药学典籍中均有详细记载，在汉代《本经》中被归为上品[2]，中医临床多用于治疗血瘀不通、身体疼痛等症状[3]。（剩余8437字）