调落物添加对蒿类荒漠草地土壤酶活性及其化学计量比的影响

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中图分类号：S812.2 文献标识码：A 文章编号：1007-0435（2026）02-0643-1

Abstract： This study investigated the effects of different addition levels （O，2.5，5.0， 7.5g ）ofSeriphidium transiliense leaf litter on the enzyme activities and stoichiometric ratios of β-1，4 -glucosidase（βG），N-acetyl- β -glucosaminidase（NAG），leucine aminopeptidase（LAP），and alkaline phosphatase（AP） in 100.0g of soil from Sagebrush desert grassland during 320 d （ ΓT1） ，410 d （T2） ，and 500 d（ T3 ）of decomposition using an outdoor Mason jar incubation method.The results showed that enzyme activities generally decreased over the decomposition period.Specifically，the βG activity in the 5.0g treatment at T2 was significantly higher than that at T1 and T3 by 12.16% and 20.53% ，respectively P<0.05） .TheNAG activity in the 7.5g treatment at T1 was significantly higher than that at T2 and T3 by 19.19% and 19.10% ，respectively （P<0.05） Enzyme activities initially decreased and then increased with increasing litter addition. At T3 ，the 7.5g treatment significantly enhanced NAG activity while reducing LAP activity （P<0.05） .The average soil enzyme stoichiometric ratios of C：N ，C： P，and N：P were O.65，O.91，and1.41，respectively，with a C：N：P stoichiometric ratio of 1： 1. 26： 1. O5，with the vector angles less than 45∘ . In conclusion，soil enzyme activities and their stoichiometric ratios were co-regulated by both liter addition amount and decomposition stage，and were constrained by soil nitrogen limitation.

Key words：Litter;Sagebrush desert grassland ;Stoichiometric ratio;Soil enzyme activitie

凋落物指植物在生长发育过程中由地上部分产生并返回到地面，为分解者提供能量和物质的有机残体，是植物地上部分与土壤进行养分交换的关键"桥梁”[1-3]，其在推动陆地生态系统养分交换与能量流动方面起到了重要作用，主要功能包括维持土壤养分平衡，涵养水源，维护生物多样性等方面[4-5]。（剩余20468字）