青绿苔草CbSH4基因克隆及基因编辑靶点筛选
打开文本图片集
中图分类号：S688.4；Q943.2 文献标识码：A 文章编号：1007-0435（2026）02-0437-10
Abstract：Carex breviculamis isan important native species that is water-saving and drought resistant，with advantages such as long green period，shade tolerance，and high fruiting rate. However，in the actual harvest ing process，the problem of seeds easily faling offlimits the mechanization of seed harvesting and large-scale production applications. In order to explore the function of SH4 gene in the process of seed-shattering in C .breviculmis and further use gene editing technology to create new germplasm resistant to seed shatering，the CbSH4 gene was cloned and the gene editing target was screened. The CbSH4 encoded 319 amino acid residues，was a hydrophilic protein，and was most closely related to Carex litledalei，containing a highly conserved Myb4 DNA-binding domain，which belonged to a typical MYB transcription factor.The transient expression results of Nicotiana benthamiana showed that CbSH4 protein was localized in the nucleus and cyto plasm.The real-time fluorescence quantification results showed that the relative expression levelof CbSH4 in the spike was significantly higher than that in the rootsand leaves，and it was expressed inthe abscisson zone at different developmental stages. At the same time，in order to achieve efficient gene editing in C . breviculmis， three specific targets were designed in this study，and sgRNA1 was obtained by sgRNA transcription and screening in vitro with a cleavage efficiency of 58.3% ，which could be used for subsequent gene editing of C breviculmis. In this study，CbSH4 was successfully cloned and the preliminary functional analysis was completed，and the high-eficiency gene editing targets were screened，which provided a basis for the creation of new germplasm with low seed shattering.
Key words： Carex breviculmis; SH4 gene;Subcellular localization; Expression analysis ; Gene target screening; Seed shattering
青绿苔草（Carexbreuiculmis）为莎草科苔草属多年生草本，具有自繁能力强、耐阴、耐旱、耐瘠薄等优点，可作优良牧草和草坪地被植物[1-2]。（剩余18135字）
