基于斑马鱼模型对Betukladin降血脂活性与机制分析

打开文本图片集

中图分类号：R961 文献标志码：A 文章编号：1002-4026（2026）01-0043-10

开放科学（资源服务）标志码（OSID）

Analysis of the lipid-lowering activity and mechanism of

Betukladin based on a zebrafish model

DU Xingshuoä， ZHAN Wena，GAO Songyinga， SUN Changgaoʰ，XU Mingxianʰ， ZHANG Sichenb，LI Hongliangb，XIA Qinga，HE Qiuxiaa，b *

（a.ShandongProvincialEngineeingResearchCenterforZebrafishModelsofHuman DiseasesandDrugScreening，InstituteofBiology;b.High-Tech Industry（PilotTest）Base，Qilu UniversityofTechnology（Shandong Academyof Sciences），Jinan，China）

Abstract ： Betukladin is a dietary supplement composed of reindeer lichen powder and birch bark extract，possessing variouspharmacologicalactivitiessuchasbloodglucoseregulation，lipidlowering，anti-inflammatoryeffcts，and anticoagulation.This studyinvestigates thelipid-loweringactivityandunderlyingmechanismof Betukladin，aiming to validateits eficacyand provideascientific basis fordeveloping safeandeconomical naturalipid-lowering drugs.Using a zebrafishhyperlipidemiamodel，wefoundthatBetukladinsignificantlyimprovedtheelevatedOiRedOstainingintegrated opticaldensityvalues，triglyceride levels，andtotalcholesterol levels inducedbyhigh-fatmodeling，indicatingitslipidloweringactivity.Networkpharmacologyanalysissuggestedthat Betukladinmayexertitslipid-loweringeffectsthrough multiple coordinated pathways，including lipid and atherosclerosis pathways and the PPAR signaling pathway.RT-qPCR resultsshowedthatitcanregulate lipidmetabolism-related genes（apoAl，LDLR，andANGPTL3），fatyacidsynthesis and oxidation genes（SREBF1，FASN，PPARα，andcptla，），andcholesterol metabolism-related genes（CYP7A1， HMGCR，ABCG5，and ABCG8）.

Key words ： hyperlipidemia； Betukladin；reindeer lichen； birch bark；lipid lowering；zebrafish model

高脂血症（hyperlipidemia）是一种以血浆中总胆固醇（TC）、甘油三酯（TG）、低密度脂蛋白胆固醇（LDL-C）升高或高密度脂蛋白胆固醇（HDL-C）降低为特征的代谢紊乱疾病。（剩余13117字）