基于“指纹图谱-模式识别-血清移行成分”整合策略筛选清消五味汤质量差异标志物

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中图分类号：R288 文献标志码：A 文章编号：1002-4026（2026）01-0011-10

开放科学（资源服务）标志码（OSID）

Screening quality-difference markers of Qingxiao Wuwei Decoction

using an integrated“ fingerprint-pattern recognition-serum

migration components” strategy

SHI Kaitao，LI Jun，ZHANG Qian* ，MA Yuheng （School of Pharmacy，Inner Mongolia Medical University，Hohhot Oio110，China）

Abstract： By establishing high-performance liquid chromatography（HPLC）fingerprints of Qingxiao Wuwei Decoction （QXWWD）and integrating chemical patern recognition with invivoand in vitrocomponent identificationmethods，this studyaimedtoscreen diferentialcompoundsand providereferencedataforconsistencyevaluationandthedevelopment of compoundpreparations.Thefingerprints of 18batches of QXWWDwere established evaluatedforsimilarity. Bioinformatics methods were used to analyze potential qualitymarkers.HPLC-Q-Exactive-MS was used to identify in vitro chemicalcomponentsand serummigrationcomponentsofQXwWD.Basedonthe integrated“fingerprint-pattern recognition-serum-migrant components”strategy，quality-diference markers werescreened.Atotal of 28peaks were identified inthefingerprintsofQXwWD，among which8quality-diferencemarkers，suchaschrysophanol，wereselected. Intotal，85invirochemicalcomponentsand55 serummigrationcomponentsof QXwWDwereidentified.Byfindingthe intersectionof thefingerprint-identifiedcomponentsandthoseidentifiedinvivoandinvitrousinga Venndiagram，we idenifiedchrysophanolasthecorequality-diferencemarker.Thisstudyestablishedasimpleandreproducibleanalytical method that can efectively evaluate thequality stabityand batch consistencyof QXwWD.This method provides reliable datato support processoptimization，quality-standarddevelopment，andthesubsequent developmentof this prescription. Keywords：QingxiaoWuweidecoction；fingerprint；paternrecognition;serummigrationcomponents；qualitydiferencemarkers

耐甲氧西林金黄色葡萄球菌（MRSA，methicilln-resistant Staphylococcusaureus ）对多种临床一线抗生素耐药，感染后的临床表现从无症状的鼻粘膜定殖到轻微的皮肤和软组织感染、骨关节感染、再到暴发性侵袭性疾病，如中毒性休克综合征、菌血症、脑膜炎和 MRSA 肺炎等[1-2]。（剩余12095字）