响应面优化棉花茎杆皮色素提取工艺及抗氧化研究

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中图分类号：S562 文献标志码：A 文章编号：1008-0864（2026）01-0255-11

Study on Response Surface Methodology Optimization of Pigment Extraction from Cotton Stem Bark and Its Antioxidant Activity

LI Taihe’，CHEN Ting1，FENG Jian1，LI Xin1，XU Shuangjiao²，TIAN Xinquan²，FANG Dan²， HAN Huanyong³，LIU Jiannan1*，MALei²*

（1.CollegeofandscapeandEcologicalEnginering，HebeiUniversityofEngneering，HebeiHandanO56O38，China；.Natioal KeyLaboratoryofCottn BiologicalBreedingandComprehensive Utilization，CottonResearch Insitute，ChineseAcademyof Agriculturaliences，Henyang455ooia；3.otonResearchIstitute，ijangcademyoficulturalie， Xinjiang Shihezi ，China.）

Abstract：Tooptimizethe extraction process ofnatural pigments fromcoton stalk fibers andanalyze their antioxidant activity， the 1，1 -diphenyl-2-picrylhydrazyl（DPPH）radical scavenging activity，2，2'-azino-bis 3-ethylbenzothiazoline-6- sulfonic acid（ABTS）radical scavenging abilityand iron-reducing power were taken as the key indicators.A strategy combining ultrasonic-asisted technologyand response surface methodology was employed to optimize the extraction conditions.Theresults showed thattheoptimalpigment extractionconditions wereas follows：liquid-to-materialratio of 104.036 mL·g-1 ，ethanol content of 73.462% ，ultrasonic temperature of 59.801°C ，and extraction time of 100.056 min.Under these conditions，the extracted pigment content was 51.646mg⋅g-1 .Theantioxidant testindicated that this pigment hadastrong scavenging ability for DPPH，ABTS free radicalsand iron ions，and the half-maximal inhibitory concentration（ IC50 ）forDPPH was 46.201 39 μo⋅mL-1 ，while forABTS the IO 50 was 19.542 30μg⋅mL-1 ： The iron-reducing assay test showed that at a pigment content of 1mg⋅mL-1 ，its activity was almost equivalent to that of vitamin C.Aboveresultsopenednewavenues forthecomprehensiveutilizationofcotton，particularly inresourceeffcient applications and food additive development，showing great potential for future application. Keywords：natural pigment from coton stem and peel；response surface methodology；antioxidant activity; extraction process；ultrasound-assisted

棉花作为农作物纤维的主要供应源及棉副产品增值加工的重要原料，已成为中国不可或缺的经济作物之一。（剩余17026字）