旋花科4种植物叶绿体基因组密码子使用偏好性分析

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图分类号：Q78 文献标志码：A 文章编号：1008-0864（2026）01-0074-12

Analysis of Codon Usage Biasof Chloroplast Genomes in 4 Convolvulaceae Family

WANG Chong，LAN Mengjiao， XIAO Manqiu，PAN Hao，DENG Jiaqi，HOU Longying，WU Wensheng （Crops Research Institute，Jiangxi Academy of Agricultural Sciences，Nanchang 33O2Oo， China）

Abstract：Inorder toclarifythecodonusage biasofchloroplast genomein Convolvulaceaeplants，4speciesof Convolvulaceae wereanalyzed fortheirchloroplast genomecodonusagepaterns including Ipomoea batatas，Ipomoeatrifida， Ipomoeatrilobaand Ipomoeanil，and the influencefactorsofcodonbiasand theoptimalcodon wereanalyzed.Theresults showed that the GC content ofchloroplast genome coding genes in 4 Convolvulaceae family was less than 50% ，and GC，>GC 12 > GC3，which indicated that their codon was prefer to end in A/U .The effective codon numbers of the chloroplast genomes in 4 Convolvulaceae family was 48.20～51.26，andthe codonadaptation index（CAI），codon bias index（CBI）and requencyof opticalcodons（FOP）were0.157～0.170，-0.096～-0.091and0.354～0.358，respectively，whichsugestedthatthecodon usagebais waslow.ENC-plot，PR2-plotandneutralplotanalysesshowedthatthecorrelation wasnotsignificantbetweenGC 11 ， GC2 and GC，andthefrequencyofthethird codonshowedT>A，G>C，which indicated that thecodonbaisof thechloroplast genomes in4Convolvulaceaeplantswasafectedbynatural selection.37optimalcodons werescreenedoutinchloroplast genomecodonsof4 Convolvulaceae family，andmostofthemendedinA/U.Aboveresultsprovidedareferenceforsubsequent studies on chloroplast genetic engineering and genetic transformation in Convolvulaceae plants.

Keywords：Convolvulaceae family；chloroplast genome；codon；usage bias

叶绿体是植物重要的细胞器之一，是细胞内含有自身遗传物质和遗传体系的半自主细胞器[]。（剩余14557字）