基于脂质组学对不同成熟度烟叶代谢标志物筛选

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中图分类号：S572 文献标志码：A 文章编号：1008-0864（2026）01-0086-11

Qualitative Screening of Lipid Metabolites in Tobacco Leaves with Different MaturityLevels Based on Lipidomics

TANG Tao 1 ，LIN Jinquan²，ZHAO Mei³，YU Zelin'，CHEN Youwen 3 MA Yimin³，HE Chenggang1*，CHEN Yi4*

（1.Yunnan Agricultural University，Kunming 6502O1，China；2.Honghe Prefecture Company，Yunnan Tobacco Company，

Yunnan Mile 65230o，China；3.Chuxiong StateTobaco Company，Yunnan TobaccoCompany，YunnanChuxiong 675000， China；4. Yunnan Academy of Tobacco Agricultural Sciences，Kunming 65oo31，China）

Abstract：Toexplore thediffrentkeycharacteristiclipidsubstancesafecting thematurityof tobacco leaves，the lipidomics methodcombined with chromatography-masspectrometryandothermethods was used todeterminethe tobacco leaves of the flue-cured tobacco variety K326 at3 different stages：under ripe（GS），ripe（SS）and over ripe（GS）for analysis，andthekeycharacteristic lipidsubstancesafecting thematurityoftobaccoleaves werescreenedout.Theresults showedthat，among3maturitygroups，there wereatotalof176diferentiallipidmetabolites betweenGSvs QS groups， 21differentiallipidmetabolitesbetween GSvsSSgroups，and140diferentiallipidmetabolitesbetween SSvsQSgroups. 5lipiddiferential metabolites werescreenedout，including lysophosphatidylglycerol，galactoglycerol diester， phosphatidylinositol，phosphatidicacid，andphosphatidylglycerol.These5lipidmetabolitescouldbeusedasmarkers to determine different maturity stages of tobacco leaves.

Keywords：K326；tobacco leaf maturity;lipidomics；metabolic marker

烟草成熟度作为烟叶品质评价的核心指标，不仅反映叶肉细胞内源代谢物的动态平衡与化学计量学特征，更通过调控栅栏组织排列密度、质体色素转化效率、苯丙烷类代谢通量、多酚/生物碱协同效应及热裂解行为参数，系统影响烟叶的物理特性谱、感官品质图谱和工业适配性指数。（剩余17514字）