小麦TaBCAT-3A、TaBCAT-3B、TaBCAT-3D基因非生物胁迫应答分析
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中图分类号：S512.1 文献标志码：A 文章编号：1008-0864（2026）01-0022-1
AnalysisofAbiotic StressResponsesofWheat TaBCAT-3A，TaBCAT-3B， TaBCAT-3DGenes
JIAYanfeng 1，2 ，WANGWeiwei²，FANG Zhaofeng²，GUO Chunman 2 ，WANGDezhou 2 ， MA Jinxiu²，WANG Xiaoyan1*，TANG Yimiao 2* ， ZUO Jinghong2* （1.Collegeof Agriculture，YangtzeUniversity，HubeiJingzhou434023，China;2.InstituteofHybrid Wheat，BeijingAcademyof Agriculture and Forestry Sciences，Beijing 1Ooo97，China）
Abstract：Branched-chainaminoacidaminotransferase（BCAT）isakeyenzyme inthemetabolismof branchedchainamino acids，and playsavital role in plant growth，development and stressadaptation.The bioinformatics analysis wasused to identify3 homologous genes in wheat，TaBCAT-3A，TaBCAT-3Band TaBCAT-3D，which were orthologsof rice OsDIATgene.These genes exhibited high similarity to OsDIAT in terms of gene structure，amino acid sequences and protein characteristics.Subcellular localization analysis revealed that TaBCAT-3A，TaBCAT3B，TaBCAT-3D proteins primarily located in cytoplasmand chloroplasts.Promoter cis-element analysis indicated thatthepromoterregionsof TaBCAT-3A，TaBCAT-3Band TaBCAT-3D were enriched with elementsresponsive to hormones and abiotic stresses.Notably，the promoter region of TaBCAT-3A containedunique defense and stressresponsive elements，furthersupportingitspotentialroleinplant stressadaptation.Expressionanalysis demonstrated that TaBCAT-3A，TaBCAT-3B，TaBCAT-3D genes exhibited significant responses under various abiotic stresses， including low temperature，drought，high temperatureand salt stress，with pronounced upregulation under lowtemperature and salt stress conditions.Comparative expressionanalysis underdrought stress in 3drought-sensitive and 3 drought-tolerant wheat materials revealed contrasting expresson paterns.In drought-tolerant materials，the expressionsof TaBCAT-3A，TaBCAT-3B，TaBCAT-3Dwere significantly downregulated，whereas TaBCAT-3A expresionindrought-sensitivematerialswassignificantlyupregulated，withnosignificantchanges inTaBCAT-3Band TaBCAT-3D.In conclusion，TaBCAT-3A，TaBCAT-3B，TaBCAT-3D genes were responded under various abiotic stress conditions，highlightingthepotentialroleofTaBCAT-3Aindroughtstressresponse.Aboveresultsprovidednew theoretical insights and practical guidance for wheat breeding improved stress resistance.
eywords：wheat；BCAT genes；bioinformaticsanalysis；abiotic stress；expression pattern
非生物胁迫是全球农业生产面临的主要限制因素之一，包括干旱、盐碱、高温和低温等环境胁迫，严重影响作物的生长、发育和产量。（剩余16769字）
