香豆素类化合物对南方根结线虫的杀线虫活性评价

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关键词：南方根结线虫；香豆素；6-甲基香豆素；杀线虫活性

中图分类号： S482.2+93 文献标识码：A 开放科学（资源服务）标识码（OSID）：

文章编号：2097-1354（2026）01-0083-10

Evaluation of Nematicidal Activity of Coumarin Compounds Against Meloidogyne incognita

XI Daojiang1， LIU Ting²， LIU Dongyang²， JIANG Lianqiang²，

LONG Gang²， REN Bo1， YANG Pinglong1 ，WEN Chengzhi1， YU Jiamin³

1.College of PlantProtection，Southwest University，Chongqing 40o715，China； 2.LiangshanPrefectureCompany，SichuanProvincial Company， ChinaNational Tobacco Corporation，Xichang，Sichuan615ooo，China； 3.SichuanProvincialTobacco ScienceResearch Institute，Chengdu61oooo，China

Abstract： Meloidogyne incognita is one of the plant obligate parasitic pathogenic nematodes， which is seriously harmful to tomato and tobacco. In recent years，with the increase of pesticides application， the resistance of Meloidogyne incognita to pesticides has gradually increased， and it is necessary to screen new substitute agents as alternative nematicides. This study evaluated the bioactivity of 1l common coumarin compounds against M . incognita through bioassays. Root galls from tobacco roots were excised and incubated to prepare a nematode suspension at a concentration of 5O individuals/ 100μL . The number of dead nematodes treated with different compounds was microscopically examined in 96-well plates after 24h and 48h of treatments.Bioassay results showed that coumarin and 6-methylcoumarin exhibited excellent bioactivity against M . incognita at 125mg/L ，with 24hLC50 value of 19.22mg/L and 39.44mg/L ，respectively. In the field experiment， Treat Cl significantly increased the plant height and maximum leaf length during the vigorous growth stage of plants，as wellas the plant height， stem diameter，and number of effective leaves during the topping stage. At the end of Treat Cl and Treat C3 had the lowest disease index （23.69 and 25.48，respectively），while the control group had a disease index of 41.83. The corresponding control efficiency was 43.37% and 39.09% ，respectively. In this experiment，coumarin and 6-methylcoumarin were screened out as safe and efficient nematicides，with good application prospects.

Key words： Meloidogyne incognita ; coumarin; 6-methylcoumarin; nematocidal activity

南方根结线虫（M.incognita）是一种专化性寄生线虫，分布广泛[1]，危害严重。（剩余12904字）