广东香水柠檬果实白皮病病原鉴定

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中图分类号：S476.1 文献标识码：A文章编号：2097-1354（2026）01-0051-06

Abstract： To determine the types of pathogenic fungus of causing suspected white peel disease on the fruits of the in Guangdong Province，isolated and identification the diseased fruits were collected by using the conventional tissue isolation method. Based on the phylogenetic analysis jointly constructed from the ITS，LSU and TUB2 gene sequences，the results showed that the NMoo5 strain isolated from Ci on fruits clustered with Didymella americana on an evolutionary branch，with a support rate of 82% on the maximum likelihood phylogenetic tree and 88% on the MrBayes phylogenetic tree.At the same time，the path Ogenicity of the isolates was determined according to Koch's rule，and the result showed that this strain could cause diseases in healthy fruits，and the symptoms were similar to those in the field.The strains re-isolated from the inoculated diseased fruits were consistent with the morphological characteristics of the inoculated fungi， indicating that the original isolated fungus were the pathogenic fungi causing white peel disease on fruits. Through morphological and molecular biological identification，the isolated strain was identificated to be Didymella americana.The results of this study provide a theoretical basis for the field control of white peel disease on fruits and ensures the healthy development of the perfume lemon industry.

Key words： ，white peel disease， pathogen fungus，Didymella americana ， pathogenicity identification

香水柠檬（Citrus×limon‘Rosso'）是一种重要的经济作物，属芸香科柑橘属常绿小乔木，果实长圆形，无核，在我国台湾、广东、云南等地均有种植。（剩余4935字）