PRDX4在肺腺癌中功能及机制分析

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关键词：肺腺癌；过氧化物酶4；增殖；细胞周期；迁移中图分类号：R734.2 文献标志码：A 文章编号：1672-1098（2025）06-0100-09

Functional and Mechanistic Analysis of PRDX4 in Lung Adenocarcinoma HU Dong12，LIU Ziqin'，ZHOU Jiawei'，LI Yunyun'，YANG Xuelian'， WU Jing'

（1.Medical CollgeofAnuiUniversityofScienceandTechnology，HuaianAnhui2321，China;.TeFrstAfiliatedHospital ofAnhui University of Science and Technology， Huainan Anhui 232oo1， China）

Abstract： Objective To investigate the impact of Peroxiredoxin4 on the tumor immune microenvironment of lung adenocarcinoma （LUAD）and to elucidate the effects of PRDX4 on tumor cellproliferation， migration， and the cell cycle，as well as its mechanisms of action.Methods By downloading sequencing data from 33 types of tumor tissues，the correlation between PRDX4 and tumor purity， immune score， stromal score， and RNA stemness score （RNAss） expression was analyzed. Immunofluorescence （IF） was used to detect the expression levels of PRDX4 in normal and tumor tissues of LUAD patients. Cell cloning，scratch，and Transwell assays were used to examine the effects of PRDX4 on tumor cellproliferation， migration，and invasion. Flow cytometry and Western blot （WB） were used to investigate the efects of PRDX4 on tumor cell cycle and epithelial-mesenchymal transition （EMT），as well as the underlying molecular mechanisms.Results Correlation analysis showed that

PRDX4 was positively correlated with tumor purity and RNAss， but negatively correlated with immune and stromal scores. IF analysis demonstrated high expression of PRDX4 in tumor tissues of LUAD patients. Functionally， the knockdown of PRDX4 significantly inhibited tumor cell migration，invasion，and cell cycle progression. Regarding the mechanism， PRDX4 may promote tumor cell EMT by regulating the AKT signaling pathway. Conclusion High expression of PRDX4 in lung adenocarcinoma may be a key regulator that promotes tumor cell migration， invasion and progression.

Key words： lung adenocarcinoma; peroxiredoxin4; proliferation; cell cycle; migration

PRDX4是一类重要的抗氧化还原蛋白酶1，先前研究发现，PRDX4在细胞内具有重要的抗氧化功能，能催化过氧化氢等氧化物的还原反应，从而清除细胞内的氧化应激，保护细胞免受氧化损伤[2]。（剩余13695字）