黏土矿物对枯草芽孢杆菌吸附特性与机制差异

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中图分类号：X172 文献标志码：A 文章编号：1672-1098（2025）06-0031-10

Abstract： Objective To investigate the adsorption behavior of Bacillus subtilis on montmorillonite and kaolin， comparing the adsorption characteristics of these two clay minerals.Methods Batch adsorption experiments were conducted to investigate the adsorption capacity of montmorilonite and kaolin for Bacillus subtilisunder different bacterial concentrations， temperatures， adsorption times， and pH conditions. The adsorption behavior was characterized by using X-ray photoelectron spectroscopy （XPS）， Fourier transform infrared spectroscopy （FTIR），and scanning electron microscopy （SEM）. Results Bacilus subtilis was rapidly adsorbed by both clay minerals， with montmorilonite exhibiting a higher adsorption capacity than kaolin. The adsorption capacity for Bacillus subtilison both clay minerals decreased with increasing pH and temperature.Notably， the adsorption on montmorilonite was more significantly affected by temperature. Conclusion The differences in the adsorption mechanisms between the two types of clay minerals at the crystal structure level are revealed.The adsorptionon kaolin primarily relies on the coordination of surface Al-OH groups， whereas that on montmorillonite is dominated by ion exchange and physical adsorption， providing more active sites to its interlayer cations and larger specific surface area.

Key words： Bacillus subtilis; kaolin; montmorillonite; adsorption mechanism

黏土矿物是地球土壤层中最主要且最活跃的组分，而土壤微生物则是地表生态系统中物种多样性最丰富、功能最复杂的群落，矿物-微生物的相互作用广泛存在于土壤圈层中[1-2]。（剩余13128字）