红景天治疗高原缺氧性脑损伤的作用机制及实验验证

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AbstractObjective：Toexplorethemechanismof RhodiolaCrenulatafortreatingplateau hypoxicbraininjurybasedonreverse moleculardockingandexperimentalverification.Methods：ThechemicalcomponentsofRodiolaCrenulatawerecollctedbysaching thecomprehensiveChinesemedicinedatabase（ETCM），thedatabaseoftheasociationbetweenChinesemedicineanddisease symptoms（Syp）ndoughpplemetariarecltioctigredntsitbotiodistrtioeabs excretion（ADME）predictionresultswerescreenedusingthedrugscreeningplatform（FAFDrugs4）.Thepotentialtargetsofactive ingredientswerepredictedbyreversemoleculardockingmethod.Tetargetsrelatedtoplateauhypoxicbraininjurywereotainedfrom thehumangenedatabase（GeneCards）.Thecommontargetsofmedicinalmaterialsanddiseaseswereobtained.The"traditionalChinese medicie-component-target-disease"regulatorynetworkandthecommontargetprotein-proteininteraction（PPl）networkwere constructed.Thecoretargetswereobtainedthroughnetworktopologyanalysis.Geneontology（GO）andKyotoencyclopediaofgenesand genomes（KEGG）enrichmentanalyseswereconductedusingtheOmicSharesoftware，andinvivopharmacodynamicexperimentswere cariedutforverfiatioeegulatoryfectofodiolareulataonthexpressooforetargetmesenerboucleicacidmRN） inthehippocampaltissueofatswithplateauhypoxicbraininjuryasdetectedbytea-timefluorscentquantativepolymeasehan reaction（RT-PCR）WestemblotwasusedtodetecttheregulatoryefectofRhodiolaCrenulataontheproteinexpresionoferine/ threonineproteinkinase1（AKT1）andsignatransducerandactivatoroftranscription3（STAT3）inbraintisueofratswithlow-presure oxygencombinedwithlipopolysaccharide（Linducedraininjuryfurthrcofiingthesutsofetworkarmacoloaalyis. Resuts：Atotalof76activecomponentsand285targetswerescreenedfromRhodiolaCrenulataOnehundredandfortyightdisease targetsrelatedtolateuhoicaiijureaieditomontargetsoretagetsiudedatsockein 90α1 （HSP90AA1），albumin（ALB），estrogenreceptor1（ESR1），andSTAT3.GOenrichmentanalysisyielded1607entries，andKEGG enrchmentanalysisidentied71pathways.RhodiolaCrenulatacoulddown-regulatethemRNAexpresionlevelsof thecoretargets serine/AKT1andSTA3.RhodiolaCreulatacouldinhibitthephosphoryltionofserine/AKT1proteinndadosescouldsignificantly inhibit the expression of STAT3 protein（ P<0.01 ）. Conclusion：Rhodiola Crenulata could treat plateau hypoxic brain injury through multitarget and multi-pathway.

Keywordsplateau hypoxic brain injury; Rhodiola Crenulata; reverse molecular docking; mechanism

随着我国经济的发展和人民生活水平的提高，高原特色旅游业得到了快速发展[1]。（剩余13990字）