基于网络药理学和实验研究探讨芪精慧心方对心脏的保护作用

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TheProtective Efectof Qijing Huixin Formulaonthe HeartBased on NetworkPharmacologyand Experimental Study LI Lin，SUN Yuanlong， ZHAO Zhejun， RUANXiaofen

ShanghaiUniversityofTradionalCineseMedicineAfliatedSuguangHospial，SubcenterofNationalClinicalResearchCeterfor Chinese Medicine Cardiology，Shanghai ，

CorrespondingAuthorRUANXiaofen，E-mail：rxfzdl @ 163.com

AbstractObjective：ToinvestigatetheprotectiveefectofQjingHuixinFormulaontheheartbasedonnetworkpharmacologyandbasic study.Methods：ThepotentialactivecomponentsofQjingHuiinFomulaanditsorespondingtargetsiteswerescreenedouthough thedrugtargetpreditionwebsiteandbyreviewingtheliteratureUsingthediseasedatabase，therelatedtargetsitesofacutemyocardial infarction（AM）wereretrievedandtheintersectionofthetwotargetsiteswasainedTeinteractionnetworkdagramofdugactive componentsandtargetsites，theprotein-proteininteraction（PPl）networkdiagramweredraw.Thegeneontology（GO）functional enrichmentanalysis，Kyotoencyclopediaofgenesandgenomes（KEGG）pathwayenrichmentanalysiswereconductedandmolecular dockingwasusedforverification.ThetherapeuticefectandmechanismofQijingHuixinFormulaintreatingAMratswereverified. Results：QijingHuiinFormulafortreatingAMinvolved39drugtargets.heuercetinaempferol，digitoninfaoids， β -glucoside，and Tanshinone II A showed the greatest effect.Protein kinase B1（AKT1），glycogen synthase kinase 3β（GSK3β） ，vascular endothelial growth factorA（VEGFA）tumornecrosisfactorT），andinterleukin6（IL6）werethekeytargets.Themainenrichedpathwaysincludedthe phosphatidylinositol3-inase（PKroteininaseBKsigalingpathaysigalingpathayndapoptosisigainpathay Thecardiacfctioofatstelsofocasctaterogase）eatiniase）， and inflammatory factors（TNF- α⋅α∝ ，IL-6），microvascular density，themRNA expressions of VEGFAandthemarkersof the Pl3K/AKT/catenin β1（CTNNB1） pathway in the myocardial infarction edge zone improved by treatment of Qijing Huixin Formula（ P<0.05 .Conclusion：Qijing HuixinFormulacouldimprovemyocardialinjuryinAMIratsbyactivatingthePl3K/GSK3β/CTNNB1signalingpathwayinmyocardial tissue and promoting angiogenesis，thereby enhancing cardiac function.

Keywordsacutemyocardialinfarction；QijingHuiinForulaphosphatidylinositol3-kinaseglycogensynthasekinase3β/ateinβ1 signalingpathway;angiogenesis; rats;experimental study

2020年《中国心血管健康与疾病报告》显示，自2002年以来，我国急性心肌梗死（AMI）患病率与病死率持续增长[1]。（剩余11117字）