SOX9通过调控SLC7A11/GPX4介导的铁死亡抑制卵巢癌进展的机制研究

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【中图分类号】R737.31 【文献标识码】A

【Abstract】 Objective To investigate the role and mechanism of sex-determining region Y-box protein 9 （SOX9） in the progression of ovarian cancer. Methods The ovarian cancer cell lines with stable knockdown of SOX9 were constructed. The effects of SOX9 knockdown on the proliferation，invasion and migration abilities of ovarian cancer cellines were detected by CCK8 assay， Transwell assay， and cellscratch assay，respectively. The effects of SOX9 knockdown on ferroptosis in ovarian cancer cell lines were evaluated by the level of Fe2+ ，glutathione （GSH）， malondialdehyde （MDA）， and Western blot analysis. The effects of SOX9 knockdown on the lipid peroxidation level of ovarian cancer cel lines were observed by confocal fluorescence microscopy. Chromatin immunoprecipitation followed by quantitative PCR （ChIP-qPCR） was performed to examine the binding of SOX9 to its downstream target gene， SLC7A11. Results Knockdown of SOX9 significantly inhibits the proliferation，invasion and migration abilities of ovarian cancer cells. Meanwhile， SOX9 transcriptionally regulates SLC7A11 and activates ferroptosis via the SLC7A11/ GPX4 signaling axis. Conclusion SOX9 promotes ferroptosis in ovarian cancer cells by regulating the SLC7A11/GPX4 signaling pathway， ultimately inhibiting ovarian cancer progression 【Keywords】sOX9; Ovarian cancer; Ferroptosis

卵巢癌是女性生殖系统中最常见的恶性肿瘤之一，其发病率和致死率在全球范围内不断升高[。（剩余10409字）