普罗布考通过MT1介导Nrf2/GPX4信号轴对PCI后心肌缺血再灌注损伤及急性肾损伤的保护机制

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AbstractObjective：ToexploretheprotectiveefectofprobucolPBC）onischemia-reperfusion（l/）injuryduringpercutaneouscooay interventin（PCl）andacute kidneyinjury（AKl）.Methods：MaleSDratswererandomlydividedintothregroups：shamoperatiogroup （Sham group），I/modelgroup（/Rgroup），nd/Rmode-BCpretreatmentgroup（/RBCgroup），with10atsinachroup.Acl modelofmyocardial/（H/Rmodelmulation）iducedalinjuryitBCpretreatmentexperimentsasstablisdpriaraal tubularepithelial cells were divided into three groups：Control group（serum-free group），H/R group （serum-free + supernatant of the culture fluid after transmigration of cardiomyocytes H/R），and H/R-PBC group（serum-free + addition of the supernatant of the culture fluid after transmigration of cardiomyocytes H/R+100μmol/L of PBC）.The effects of PBC on myocardial injury and renal tissue injury were assessedby2，3，5-tripheyltetrazolumcloide（TC）stainngandmatoxylnosinHE）staining.TheefectsofPBConmyordialand renatissueijuryweredetectedbyserology.TheeffctsofPBConapoptosisofrnaltubularepithelialcellsweredetectedbyWestem BlotandflowcyometryTemAandproteinlevelsofmelatoinreceptor1，uclearfactorerythoidrelatedfactorf）d glutathioneperoxidase4（GPX4）inrenaltissuesandrenaltubularepithelialcelsweredetectedbyRT-PCR，WestemBlotand knockdowtransfectiontechqus.Resus：PBCatenuatedischemicmycardialinjuryandAKlinatsrducedrnaltsueadcelular injuryandimproedpost-ischemicmyocardialandnalfunctioPBCrducedapoptosisofenaltbulareithelilcellandupreulated MT1Nrf2/GPX4.ConusioBCprecondiongateuates/RinjuucedAKbymediatingtheNf/GPX4signalingaisthh1. Keywordspercutaneouscoronaryintervention;myocardialishemia-eperfusion;acute kidneyinjury;probucol; experimetalstudy

过去10年经皮冠状动脉介入治疗（PCI被临床广泛应用，但急性心肌梗死的死亡率（约为 5% ）并未得到降低[1-2]。（剩余12212字）