微波功率-温度协同调控下的多肽固相合成

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文章编号：1003-9015（2026）01-0071-11

中图分类号：0629.72 文献标志码：A DOI：10.3969/j.issn.1003-9015.2025.00.038

Solid-phase peptide synthesis under microwave power-temperature synergistic control

LIFei，YANGXu， SUXianbin （College of Chemical Engineering，Nanjing Tech University，Nanjing 210o09，China）

Abstract：This study focuses onexploring the synergistic interaction efect between temperature fields and electromagnetic fields during microwave-assisted solid-phase peptide synthesis （SPPS）.A self-developed microwave irradiation-temperature control circulation system was employed to achieve precise regulation of both microwave power and reaction temperature. Under continuous microwave irradiation，the kinetics of peptide chain coupling and 9-fluorenylmethoxycarbonyl（Fmoc）deprotection were investigated，establishing optimal reaction durations （ 3.5min for coupling and 2.5min+2.5 min for deprotection）. Based on these parameters，a model system featuring a representative difficult sequence in SPPS was selected.The synthesis performance under three diffrent heating modes—continuous microwave， pulsed microwave （duty cycle 0.6），and conventional water bath—was compared under equivalent temperature conditions. The results demonstrated that continuous microwave irradiation significantly improves product purity and reduces the duration of key reaction steps by approximately 70% .These findings provide a novel engineering solution for the efficient synthesis of complex peptides.

Key words： peptide； synthesis； heat transfer；microwave-asssted synthesis； solid-phase synthesis

1前言

目前，微波技术在多肽合成领域的应用价值已经得到了广泛验证[1-2]。（剩余14556字）