重组大肠杆菌转化 L. -阿拉伯糖合成木糖醇

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中图分类号：Q819 文献标志码：A DOI： 10.3969/j.issn.1003-9015.2025.00.007

文章编号：1003-9015（2026）01-0063-08

Abstract： ： L -Arabinose is a crucial component of hemicellulose hydrolysate. In the chemical production process of xylitol，it is often discharged along withthe xylose mother liquor，leading to resource waste and environmental pollution. In this study， a biosynthetic pathway for converting L -Arabinose into xylitol was established in Escherichia coli W3110. A multicistron expression module containing three enzymes required for xylitol biosynthesis，namely DPE，AraA，and LXR，was constructed.Meanwhile， the araBAD and lyxK genes in the L 1 Arabinose metabolic branch pathway of the E coli W3110 genome were knocked out， and the xylitol- producing strain ΔW -DLA was successfully obtained. Shake-flask fermentation was carried out using 10g⋅L-1 ， L -Arabinose as the substrate and 10g⋅L-1 glycerol as the co-substrate. The results showed that after 30h of fermentation， the xylitol yield reached 8.26g⋅L-1 ，and the conversion rate of L -Arabinose was 92.3% . The culture medium of the engineered strain ΔW -DLA was optimized through single-factor experiments and response-surface experiments. After optimization，△W-DLA produced 9.46g⋅L-1 xylitol after 24 hours of fermentation，and the xylitol production rate was increased by 39.3% compared with that before optimization. This study provides a new way for the resourceutilization of xylose mother liquor.

Key words： L -Arabinose； Escherichia coli； xylitol； response surface optimization

1前言

木糖醇是一种五碳糖醇，常作为甜味剂添加于糖果、糕点、饮料等食品中。（剩余11551字）