芳樟醇合酶基因(LIS)在薰衣草萜类物质合成中的功能分析

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中图分类号：TQ654

文献标识码：A

文章编号：1000-4440（2026）04-0808-09

Functional analysis of linalool synthase gene （LIS） in terpenoid synthesis of lavender

ZHANG Minghao， WANG Aifan， GONG Lintao， SU Xiujuan

（College of Agronomy， Xinjiang Agricultural University/Xinjiang Key Laboratory of Crop Biological Breeding/Lavender Research Institute， Xinjiang Agricultural University， Urumqi ， China）

Abstract： To investigate the function of the linalool synthase gene （LIS） in the regulation of terpenoid metabolism in lavender， Agrobacterium-mediated genetic transformation was used to modify the leaves of lavender （Lavandula×intermedia）， generating lavender plants overexpressing the LiLIS gene. By analyzing the expression levels of genes involved in the terpenoid biosynthesis pathway and changes in terpenoid compound content， the functions of the LiLIS1 and LiLIS2 genes in lavender were verified. The results showed that， compared with wild-type （WT） lavender， overexpression of the LiLIS1 and LiLIS2 genes significantly increased the content of the monoterpenes linalool and lavandulol in lavender （P<0.05）. Additionally， carotenoid content in plants overexpressing LiLIS1 and LiLIS2 was increased to varying degrees. However， the content of monoterpene lavandulyl acetate decreased to varying degrees. The content of camphor decreased significantly in plants overexpressing the LiLIS2 gene （P<0.05）. Therefore， the LiLIS gene plays an important role in the metabolism and biosynthesis of terpenoids in lavender. These findings can provide a theoretical basis and genetic resources for the improvement of lavender cultivars.

Key words： linalool synthase gene （LIS）; lavender; terpenoids

芳樟醇是一种挥发性单萜化合物，存在于多种芳香植物的精油中，具有清甜的花香和果香气味，是香精、香水的重要成分[1]，同时芳樟醇还有抗菌[2]、消炎[3-4]、抗氧化[5]等多种生物活性。（剩余14225字）