超甜玉米籽粒维生素 C 含量关联位点定位及基因挖掘

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中图分类号：S513

文献标识码：A

文章编号：1000-4440（2026）04-0682-09

Association mapping and gene mining for vitamin C content in super-sweet corn kernels

DENG Zhao， CUI Nini， YU Yulin， WEN Weiqi， CHENG Xinxin， YU Haibing （College of Agriculture， Anhui Science and Technology University， Fengyang 233100， China）

Abstract： To uncover the regulatory mechanisms underlying the accumulation of nutrients in the kernels of super-sweet corn， this study employed the BLINK model to identify the major loci regulating vitamin C content in super-sweet corn kernels and predict the candidate genes. The results showed that the vitamin C content in super-sweet corn kernels ranged from 0.156 mg/g to 1.289 mg/g， with an average of 0.749 mg/g， exhibiting a normal distribution. A genome-wide association study （GWAS） identified six single nucleotide polymorphism （SNP） loci significantly associated with kernel vitamin C content， with the phenotypic variation explained （PVE） ranging from 9.53% to 12.83%， among which the Affx-91328273 locus had the highest PVE. A total of 153 candidate genes were identified within the 200 kb confidence interval upstream and downstream of the significant SNP loci， of which 58 were annotated genes， accounting for 37.9%. Six genes including GRMZM2G093098， GRMZM2G106061， GRMZM2G179308， GRMZM2G101274， GRMZM2G004222 and GRMZM2G133631 were identified as key genes regulating kernel vitamin C content in super-sweet corn. These genes were mainly involved in cell division， signal trans- duction， energy metabolism and other pathways. These findings provide important support for an in-depth understanding of the genetic mechanisms underlying kernel vitamin C content in super-sweet corn.

Key words： super-sweet corn; vitamin C; genome-wide association study; candidate genes

甜玉米（Zea mays L. saccharata Sturt）是玉米属的甜玉米类型，以甜美口感和鲜嫩质地著称 [1] ，其甜质性基金项目：安徽省高校自然科学研究重大项目（KJ2019ZD57）;安徽科技学院科研发展基金项目（FZ230126）;安徽省功能农业与功能食品重点实验室（安徽科技学院）项目（iFAST-2024-3）;安徽科技学院作物学高峰学科基金项目（XK-XJGF001）状主要由 su1，su2，sh1，sh2，sh4，bt1，bt2，se，ea，wx，du，Ae 等隐性基因调控 [2]。（剩余14231字）