整合多芯片数据分析预测动脉粥样硬化进展中免疫浸润相关诊断基因生物标志物的研究

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ThePredictionofAtherosclerosisProgressionbyDiagnosticGeneBomarkersAssociatedwithImmunelfltrationofviaIntegated Multi-array Data Analysis

YOU Hongjun，DONG Mengya，CHANG Fengjun，GOU Qiling，HAN Wenqi

ShaanxiProvincialPeople'sHospital，Xian71oo61，Shaanxi，China Corresponding Author HAN Wenqi，E-mail： hwq315@163.com

AbstractObjectiveToidentifythepotentialgenesinvoledintheprogressionofatherosclerossandexploretheasoiationbetween genesandimmuneclinfitrationMethods：Twotranscriptoeprofle（GSE28829andGSE41571）wereanalyzdusingRpackagesfor diferentialgeneexpresion，geneontology，patwayanddiseaseontologyenrichment，protein-proteininteractionandcadidate biomarkerselectio.Teexpresionevelsofandidatebomarkerswereverfiedinavalidationdatasetndthecompostioalpaesof immunecelfactionswerecalculatedusingClBERSORT.Thecorelationanalysisetweendiagnosticgenebiomarkersandinfitrating immunecellswasperformedusingSpearman'srankcorelationanalysisResut：Atotalof15genesweredeterminedasdiagnosticgene biomarkersofdotisceaii CD14 and HCKwereidentfiedas hubgenes，whichwerepositivelycorelated with macrophage M2andnegativelycorrelatedwith CD8+T cell and activateddendritic cell.Conclusion：C1QA，C1QB，C1QC，LY86， CD14 and HCKwere determined as hub geneswhich were identiedtoregateimmunecompetencelTsefindingsevealightontgensasatedwitimmunecellineprogesonf atherosclerosisand may aid the development of new treatmentsand medications.

Keywordsatherosclerosis;progression; diagnostic gene biomarkers;immune infiltration;bioinformatics

动脉粥样硬化（atherosclerosis，AS）是一种慢性、进行性和炎症性的动脉疾病，是全球发病率和死亡率最高的原因之一[1]。（剩余15414字）