太白贝母种子低温萌发过程中C3H基因家族鉴定及表达分析

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中图分类号：Q943 文献标识码：A 文章编号：1000-3142（2026）01-0110-13

Identification and expression analysis of C3H gene family during low-temperature germination of Fritillaria taipaiensis seeds

YUAN Yuan1²，DONG Jiabang12， XU Yanjiao1²，LI Yan1²， ZHANG Xiaoyu12，WU Liang1，²，CHEN Yongsheng³，LIANG Yanli1，2 *

legeofAgricuredotecoogaAcuralUesityuin5Oi;toldcalJo Research CenterforGermplasmInnovationandUtilizationofSouthwestChineseMedicinalMaterials/KeyLaboratoryofMedicinal Plant Biology in Yunnan Province，Yunnan Agricultural University，Kunming 65O2o1，China;3.Lijiang Lzhiuan Biotechnology Pharmaceutical Technology Development Co.，Ltd.，Lijiang 6741oo，Yunnan， China）

Abstract：Toanalyze the roleof C3H genesduring the germination ofFritillaria taipaiensis seeds，this study utilized low-temperature germination transcriptomedataand bioinformaticsmethodstosystematically identifyandanalyze the expression of the C3H gene family in F .taipaiensis.Five low-temperature candidate genes with high expression were identified and their response mechanisms in response to gibberellic acid （ GA3 ）and abscisic acid（ABA）were elucidated.The resultswere as follows：（1）Among 65 identified C3H familymembers，7were clasifiedas stable hydrophilic proteinsand58 exhibited instability，with significant variation in protein length （197-1451aa）.Only FtC3H43andFtC3H5O werelocalizedtochloroplast/endoplasmicreticulum，whiletheotherswerenuclearlocalized.（2）Conservedmotif analysis revealed that Motif1andMotif3werewidelypresentinC3H proteins. Phylogenetic analysis results showed that196 C3H proteins were clustered into five distinct clades（I-V），with F taipaiensisC3Hsshowing close evolutionaryrelationships tothoseinArabidopsis thalianaandOryzasativa.（3）Under （204号 GA3 treatment，FtC3H22 and FtC3H35 promoted germination during early-to-mid stages （A-C），while FtC3H40 and FtC3H46 exhibited stage-specific expression at stages AandE，respectively.ABA treatment significantlyupregulated FtC3H46 but downregulated FtC3H22 and FtC3H35，suggesting FtC3H46 might mediate ABA-dependent cold stress responses，whereas FtC3H22 and FtC3H35 played positiveroles in germination regulation.This studyreveals the important roleand complex regulatory mechanism of the C3H protein family in the seed germination of F .taipaiensis.

Key words：Fritillaria taipaiensis，C3H，seed germination，bioinformation analysis，expression patter

川贝母是我国珍稀名贵中药材，具有润肺止咳、化痰平喘、散结消肿等功效（刘梓贤等，2022）。（剩余12777字）