贝莱斯芽孢杆菌G127的高密度发酵工艺优化

打开文本图片集

中图分类号：S476 文献标志码：ADOI：10.13338/j.issn.1674-649x.2025.01.003

Optimization of high-density fermentation process for Bacillus velezensis G127

ZHANG Genlin1 ，HU Kexin1 ，ZHOU Chao1 ，DU Xiaopan1 ，XU Shiwu² ，GAO Bo²

（1.College of Chemistry and Chemical Engineering/Key Laboratory of Green Process Corps of Chemical Engineering，Shihezi University，Shihezi 832oo3，Xinjiang，China; 2.Woda Agricultural Technology Co.Ltd./Key Laboratory of Agricultural Microbiology and Pharmaceutical Fertilizer Creation Corps，Shihezi 8320o3，Xinjiang，China）

Abstract Bacillus velezensis is widely distributed and has high biological activity，which is a safe and reliable biocontrol strain.Reducing cost and increasing cellbiomassis the key to its wide application.The optimal carbon source，nitrogen source and inorganic salt were selected by single factor experiment，and then the high-density fermentation conditions and process of Bacillus velezensis Gl27 were optimized by response surface. The optimal medium composition and culture conditions were as follows：beef paste supplemental level 15.2g/L ，molasses 13.0g/L ，sodium chloride 5.0g/L ，fermentation time 72h ，fermentation temperature 30∘C ，inoculation amount 3.3% ，and initial pH value 5.1. The optimized biomass could reach 5.45×1010 CFU/mL. The obtained high-density fermentation technology provides guarantee for the large-scale production and application of Bacillus velezensis G127.

Keywordsbiological control; Bacillus velezensis； response surface optimization; high-density fermentation process

0引言

生物防治是一种以生物学手段控制农业、林业、园艺和草原草甸等领域农作物病虫害的方法，其环境效益高，可利用自然资源丰富，因此生物防治技术控制农作物病虫害已经成为农业生产中不可或缺的组成部分[1]。（剩余12855字）